THE STUDY OF THERMAL STABILITY OF DNA DUPLEX ON NANOPARTICLE-BASED PLATFORMS: A METHOD FOR BIOSENSING APPLICATIONS
Keywords:Biosensor, Gold Nanoparticle, DNA duplex thermal stabilization, DNA detection
In this research, a new method for DNA detection is proposed by studying the thermal stability of DNA duplexes modified on the nanostructured platform. The nanostructures are fabricated by using the block copolymer nanolithography method to form ordered arrays of gold nanoparticles on the silicon substrate. The thermally induced denaturation of the DNA duplex on the sensing part is monitored to determine the heat transfer property (thermal resistance) at the solid-liquid interface. An about 50% change in the thermal resistance during the DNA denaturation has fairly confirmed the feasible working of the sensing platform. The sensitivity, selectivity, and repeatability of the DNA biosensor are also evaluated by analysing the thermal resistance behaviour during thermal induction cycles. For the first time, a label-free, cost-effective, and easy data read-out DNA sensing method is developed in comparison with conventional fluorescence techniques.